Coding

Part:BBa_K1727002:Design

Designed by: Ying Kuan   Group: iGEM15_TCU_Taiwan   (2015-09-02)


Signal peptide (S.lividans chitinase C)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had changed the originally sequence into E.coli codon usage.

To make sure the secretion system is work we attached an Ala amino acid at the end of the C-terminal.

Source

Artificial synthesis.

References

Fujii, T. and K. Miyashita, Multiple domain structure in a chitinase gene (chiC) of Streptomyces lividans. J Gen Microbiol, 1993. 139(4): p. 677-86.

Tokuyasu, K., et al., Production of a recombinant chitin deacetylase in the culture medium of Escherichia coli cells. FEBS Lett, 1999. 458(1): p. 23-6.